What is CyTOF mass cytometry?

Mass cytometry, or CyTOF (Fluidigm), is a variation of flow cytometry in which antibodies are labeled with heavy metal ion tags rather than fluorochromes. Readout is by time-of-flight mass spectrometry. A limited number of additional antibodies can be added to open channels, subject to availability.

How does a mass cytometer work?

How does a mass cytometer work? Mass cytometers analyze cells labelled with antibodies conjugated to metals, rather than fluorochromes, in a mass spectroscopy-coupled flow cytometer, also known as a CyTOF.

What does CyTOF do?

Cytometry by time of flight, or CyTOF, is an application of mass cytometry used to quantify labeled targets on the surface and interior of single cells. CyTOF allows the quantification of multiple cellular components simultaneously using an ICP-MS detector.

How does flow cytometry operate to detect cells?

Flow cytometry is a technology that provides rapid multi-parametric analysis of single cells in solution. Flow cytometers utilize lasers as light sources to produce both scattered and fluorescent light signals that are read by detectors such as photodiodes or photomultiplier tubes.

What is a CyTOF panel?

Mass cytometry (CyTOF) is a form of single cell analysis, akin to flow cytometry, that employs a modified time-of-flight mass spectrometer to measure relative protein abundance. Utilizing a 42 metal catalogue, we have developed panels for both human and mouse immune cell subsets, along with other cell classifications.

How much does a CyTOF cost?

The company is currently taking orders for the new system, which it plans to begin shipping in the third quarter of this year. George declined to give an exact price for Helios but said that it would cost around 10 percent more than the CyTOF 2, which would place it in the range of $600,000 to $700,000.

What is a flow cytometer used for?

Flow Cytometry is a technique used to detect and measure physical and chemical characteristics of a population of cells or particles. In this process, a sample containing cells or particles is suspended in a fluid and injected into the flow cytometer instrument.

Is CyTOF quantitative?

Mass cytometry (cytometry by time-of-flight; CyTOF) can detect as many as 40 markers present on millions of single cells. Here, we developed a workflow, called cytofast, for a fast and quantitative analysis of flow and mass cytometry data.

What does flow cytometry tell us?

Flow cytometry provides a well-established method to identify cells in solution and is most commonly used for evaluating peripheral blood, bone marrow, and other body fluids. Flow cytometry studies are used to identify and quantify immune cells and characterize hematological malignancies.

What does a flow cytometry test show?

Flow cytometry can identify the type of cells in a blood or bone marrow sample, including the types of cancer cells. It detects types of cancer cells based on either the presence or the absence of certain protein markers (antigens) on a cell’s surface.

Is CyTOF single-cell?

Flow cytometry has been primarily used to address single-cell behavior. The combination of the two techniques, termed single-cell mass cytometry (CyTOF), allows the simultaneous measurement of more than 40 cellular parameters at single-cell resolution with over 100 available detection channels30, 31.

What is CyTOF ready?

CyTOF® mass cytometry is an emerging and exciting application for research. CyTOF® mass cytometry utilizes rare metals tagged to antibodies, allowing researchers to examine over 30 different proteins on and within a single cell without concern of overlapping emission spectra.

What is CyTOF (mass cytometry)?

Mass cytometry for single-cell analysis was commercialized by DVS Sciences as the CyTOF, which was acquired by Fluidigm in 2014. Cells are stained with an antibody tagged with a purified isotope of a lanthanide metal, which are then resolved and quantified via ICP (qTOF) MS at a rate of >1000 cells per second.

What is mass cytometry by time of flight?

Mass cytometry, or cytometry by time-of-flight (CyTOF), is a valuable tool in the high-dimensional analysis of single cells [16]. In CyTOF antibodies are labeled with isotopes, followed by the labeling of cells, before analysis using high-throughput spectrometry.

How can I perform mass cytometry with isotopes?

To perform mass cytometry, the isotopes need to be introduced into the TOF without also contaminating the instrument with cells. This requires the vaporization of the cells, leaving only the isotopes.

How does CyTOF work?

How CyTOF Works. This requires the vaporization of the cells, leaving only the isotopes. To do this, the cells are introduced to an inductively coupled plasma (ICP); here the cells are vaporized and the metal ions are introduced into the TOF.